Part:BBa_K3257111:Design

T7 Promoter-lox5171-R-PPT-RBS-ChlR-PBS-U5-R-loxP-T7 Terminator

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 1107
  
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI.rc site found at 945
  Illegal BsaI.rc site found at 966
  

Design Notes

The lox sites, PBS and gene of interest should be changeable according to the users' demands and purposes.

Source

Composite from the registry.